a549  (ATCC)

Journal: Computational and Structural Biotechnology Journal

Article Title: Leveraging Pretrained Neural Network Models for the Classification of Tumor Cells Analyzed by Label-Free Phase Holotomographic Microscopy

doi: 10.34133/csbj.0111

Figure Lengend Snippet: Selected phase holotomographic tomography (PHT) images of untreated and treated cancer cells. (A) Representative images of untreated A549 cells (a to f). (B) Representative image of treated A549 + PTX (a to f). The nucleus is highlighted by orange arrows. Blebs and vesicles are highlighted by yellow and blue ellipses, respectively.

Article Snippet: Human lung adenocarcinoma A549 cells (ATCC, Virginia, USA) were cultured in Dulbecco’s Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin (PS).

Techniques: Tomography

Journal: Computational and Structural Biotechnology Journal

Article Title: Leveraging Pretrained Neural Network Models for the Classification of Tumor Cells Analyzed by Label-Free Phase Holotomographic Microscopy

doi: 10.34133/csbj.0111

Figure Lengend Snippet: Refractive index distribution for A549 cells (gray) and A549 + PTX cells (red), using only segmented cells to avoid averaging with background.

Article Snippet: Human lung adenocarcinoma A549 cells (ATCC, Virginia, USA) were cultured in Dulbecco’s Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin (PS).

Techniques: Refractive Index

Journal: Computational and Structural Biotechnology Journal

Article Title: Leveraging Pretrained Neural Network Models for the Classification of Tumor Cells Analyzed by Label-Free Phase Holotomographic Microscopy

doi: 10.34133/csbj.0111

Figure Lengend Snippet: Cross-validation confusion matrix for the EfficientNet-B0 model on A549 cells. (A) Run 1, (B) run 2, and (C) run 3. (D) Receiver operating characteristic (ROC) curve of the EfficientNet-B0 model for the cross-validation (CV) across the 3 runs, for the unsegmented dataset. Folds 1 to 5 are from run 1, folds 6 to 10 are from run 2, and folds 11 to 15 are from run 3.

Article Snippet: Human lung adenocarcinoma A549 cells (ATCC, Virginia, USA) were cultured in Dulbecco’s Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin (PS).

Techniques: Biomarker Discovery

Journal: Computational and Structural Biotechnology Journal

Article Title: Leveraging Pretrained Neural Network Models for the Classification of Tumor Cells Analyzed by Label-Free Phase Holotomographic Microscopy

doi: 10.34133/csbj.0111

Figure Lengend Snippet: Comparison of selected PHT images (top) and heatmaps (bottom) generated with Grad-CAM++ for the EfficientNet-B0 model on A549 and A549 + PTX datasets. These heatmaps were generated for the correctly predicted (A and B) “A549 + PTX” and (C and D) “A549”. All images are from the test set.

Article Snippet: Human lung adenocarcinoma A549 cells (ATCC, Virginia, USA) were cultured in Dulbecco’s Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin (PS).

Techniques: Comparison, Generated